Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages

Figure 5

Effect of aspirin on carcinogenic cytokine production by 4T1 breast cancer cells cultured in control medium and RAW-CM. (a) Effect of RAW-CM on cytokine production of 4T1 cells. Cells were cultured in 50% RAW-CM for 72 h, and then cytokines in the supernatants were measured by ELISA. (b) Aspirin was used to treat 4T1 cells, which were cultured in control medium (1% FBS/DMEM) for 72 h, and cytokine levels in the supernatants were measured. (c) Aspirin was used to treat 4T1 cells, which were cultured in 50% RAW-CM for 72 h, and then cytokine levels in the supernatants were measured. Data are shown as mean ± SEM. Statistical analysis was performed using independent sample t-tests, where statistically significant differences are indicated as , , and versus control.

(a)

(b)

(c)