Aspirin Disrupts the Crosstalk of Angiogenic and Inflammatory Cytokines between 4T1 Breast Cancer Cells and Macrophages

Figure 7

Aspirin inhibited angiogenic and inflammatory cytokines in supernatants of 4T1 and RAW 264.7 cell cocultures. 4T1 cells were cultured in the presence of macrophages for 72 h, supernatants were collected, and cytokine levels relative to tumor characteristics were measured by ELISA. (a) Angiogenic cytokines VEGF, MCP-1, and PAI-1. (b) Inflammation-related cytokines TNF-α, IL-6, TGF-β, and IL-10. The blank bar indicates RAW 264.7 cells only, the dotted bar indicates 4T1 cells only, the gray bar indicates cocultures containing both cells, and dark gray indicates cocultures treated with 2 mM aspirin. Data are shown as mean ± SEM. Statistical analysis was performed using independent sample t-tests. Statistically significant differences are indicated as , , and for treatment versus co-control vehicle.

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