Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88

Figure 2

The mRNA levels of components of epithelial barrier in IPEC-1 cells. Relative transcript levels of ZO-1 (a), occludin (b), claudin-1 (c), and F-actin (d) in IPEC-1 cells were determined by qPCR. GAPDH and β-actin were used as housekeeping genes, and the 2^–ΔΔCt method was used to determine the relative abundance. Data were further normalized to values measured in control treatments. All data are expressed as the mean ± SEM () and representative of 3 independent experiments. Differences were determined by one-way ANOVA. compared with control, .

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