Lactobacillus reuteri LR1 Improved Expression of Genes of Tight Junction Proteins via the MLCK Pathway in IPEC-1 Cells during Infection with Enterotoxigenic Escherichia coli K88

Table 1

Primers used for real-time PCR in this study.

Gene^1	Primer sequence (5-3)	Accession number
ZO-1	Forward: AGCCCGAGGCGTGTTT	XM_013993251
	Reverse: GGTGGGAGGATGCTGTTG
Occludin	Forward: GCACCCAGCAACGACAT	XM_005672525
	Reverse: CATAGACAGAATCCGAATCAC
Claudin-1	Forward: GACTCCTTGCTGAATCTGA	NM_001244539
	Reverse: GCACCTCATCATCTTCCAT
F-Actin	Forward: TCTGGAATGGTCGTTGGA	NM_001097454
	Reverse: CCTTGAATGTGGTGTCTGA
β-Actin	Forward: CTGCGGCATCCACGAAACT	XM_003124280
	Reverse: AGGGCCGTGATCTCCTTCTG
GAPDH	Forward: ACTCACTCTTCCACTTTTGATGCT	NM_001206359
	Reverse: TGTTGCTGTAGCCAAATTCA

¹zonula occluden-1