Pharmacological Inhibition of Caspase-1 Ameliorates Cisplatin-Induced Nephrotoxicity through Suppression of Apoptosis, Oxidative Stress, and Inflammation in Mice

Figure 2

Effects of Ac-YVAD-cmk on cisplatin-induced apoptotic cell death in the kidneys. (a) Western blot analysis of the expression of cleaved caspase-1 and cleaved caspase-3 in the kidneys. Graphs show the results of quantitative analysis of cleaved caspase-1 (b) and cleaved caspase-3 (c). (d) Representative images of TUNEL staining on kidney sections. Nuclei were counterstained with DAPI. Scale bar: 50 μm. (e) The number of TUNEL-positive cells in 5 random fields for each kidney. (f) Representative immunofluorescence staining of Ki67 (green) and p-H3 (red) on kidney sections. Nuclei were counterstained with DAPI. Scale bar: 50 μm. (g) Percentage of cells in the G2/M phase among all proliferative (Ki67-positive) tubular epithelial cells. Con: control, CP: cisplatin, and CP + YVAD: cisplatin plus Ac-YVAD-cmk. per group. All data are expressed as the mean ± SEM. and vs. Con. and vs. CP.

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