PICK1 Deficiency Induces Autophagy Dysfunction via Lysosomal Impairment and Amplifies Sepsis-Induced Acute Lung Injury

Figure 1

PICK1 participates in autophagic progression in sepsis-induced ALI. (a, b) TNF-α and IL-1β concentrations were measured in BALF. (c, d) Lung injury was evaluated by lung wet : dry weight ratio, and permeability was assessed by detecting changes in total BALF protein concentration. (e) Lungs were harvested at 4 h, 8 h, and 24 h post-CLP, and Western blot was performed to analyze PICK1, LC3-II/LC3-I, and p62 content. Representative Western blot images and the statistical density of PICK1 are shown in the graphs. (f, g) PICK1 and LC3 mRNA levels measured in the lungs by quantitative PCR. Results represent the mean ± SEM (/group). vs. sham-operated groups; vs. 4 h post-CLP; vs. 8 h post-CLP.

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