Levistilide A Ameliorates NLRP3 Expression Involving the Syk-p38/JNK Pathway and Peripheral Obliterans in Rats

Figure 4

LA reduced the damage to the rats’ lower limbs in the TAO model. A right lower extremity arterial injection of sodium laurate 10 mg/mL 0.2 mL was used in the positive control group, and 0.9% physiological saline 0.2 mL femoral artery injection was used in the negative control group. The effect of the operation was confirmed using laser speckle flowmetry 30 min after surgery. The rats in the control group and positive and negative control groups were given the adjuvant (CMC-Na) only, whereas the LA group received 20 mg/kg/d for 2 weeks, which started to gavage 2 d before surgery. One week after surgery, various degrees of gangrene were observed in the rats’ lower right limbs, as shown in (a). The score of the right lower limb of each group was assessed according to the TAO model score standard (b). The blood flow of the lower limbs in each group was measured using laser speckle flowmetry (c). Inflammatory necrosis of the right leg muscle was observed using HE-stained slices (400) (d). Data are presented as . versus negative control group; and versus positive control group.

(a)

(b)

(c)

(d)