Long Noncoding RNAs and mRNA Regulation in Peripheral Blood Mononuclear Cells of Patients with Chronic Obstructive Pulmonary Disease

Figure 6

Regulatory role of ENST00000502883.1 on CXCL16 expression. (a) Expression of ENST00000502883.1 on CXCL16 of 6T-CEM cells under the stimulation of 5% cigarette smoke extract (CSE). Data are presented as 2^−ΔΔCt relative to β-actin. . (b) Expression of CXCL16 on mRNA level of 6T-CEM cells with ENST00000502883.1 siRNA transfection. Scramble siRNA was used as negative control (NC). Data are presented as 2^−ΔΔCt relative to β-actin. . (c) Expression of CXCL16 on protein level of 6T-CEM cells with ENST00000502883.1 siRNA transfection. CXCL16 expression was examined by Western blotting. . (d) The regulatory role of ENST00000502883.1 on the partially CXCL16-dependent chemotactic effect of 6T-CEM cell supernatant. 6T-CEM cells were transfected with ENST00000502883.1 siRNA or negative control (NC), and the chemotactic effect of cell culture supernatant on PBMCs was evaluated by a microchemotaxis Boyden chamber. and compared with medium control. when comparing the two groups with line marks.

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