Research Article

The Kat in the HAT: The Histone Acetyl Transferase Kat6b (MYST4) Is Downregulated in Murine Macrophages in Response to LPS

Figure 2

TaqMan Q-RT-PCR assays were performed using RNA samples from untreated macrophages or macrophages treated with LPS (100 ng/ml), NECA (1 μM), or LPS (100 ng/ml) with NECA (1 μM) for 12 hours. Samples from 3 separate experiments were each analyzed in duplicate, with data indicating mean fold change relative to untreated . Expression levels were normalized to the expression of cyclophilin-D as a housekeeping gene.