Macrophage LRP1 Promotes Diet-Induced Hepatic Inflammation and Metabolic Dysfunction by Modulating Wnt Signaling

Figure 3

Regulation of hepatic glucose metabolism signaling pathways in LDLR^−/−; macLRP1^−/− mice challenged with a Western diet. LDLR^−/− and LDLR^−/−; macLRP1^−/− mice were maintained on a Western diet for 2 weeks. Livers were processed and analyzed for levels of phosphoproteins by Kinexus immunoblot (a, ). Molecular weight markers are indicated on the left. Circled bands are specific for GSK3α (1), GSK3β (2), and p38 MAPK (3). (b) Quantification of immunoblots was performed using Kinexus KCPS-1.3 phosphoprotein profiling screen software. (c) Hepatic fat content was visualized in LDLR^−/− and LDLR^−/−; macLRP1^−/− mice maintained on a Western diet for 4 weeks by hematoxylin and eosin (H&E) staining. Representative micrographs (20x) of liver sections from LDLR^−/− () and LDLR^−/−; macLRP1^−/− () mice. (d) Hepatic LRP1 expressions in LDLR^−/− and LDLR^−/−; macLRP1^−/− mice () that were maintained on a Western diet for 8 weeks. Livers were processed and analyzed for levels of LRP1 by immunoblotting. (e) Immunoblot results in (d) were quantified by densitometry using NIH ImageJ software and normalized to Hsp90 (). The data represent mean ± SEM of results. .

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