Macrophage LRP1 Promotes Diet-Induced Hepatic Inflammation and Metabolic Dysfunction by Modulating Wnt Signaling

Figure 5

LRP1 directly binds to sFRP-5 and may modulate Wnt signaling. (a) Plasma sFRP-5 levels of LDLR^−/− () and LDLR^−/−; macLRP1^−/− () mice prior to and after being maintained on a Western diet for 8 weeks. (b) Binding of increasing concentrations of sFRP-5 (1.6, 3.1, 6.3, 12.5, 25, 50, and 100 nmol/l) to LRP1 immobilized on the surface of a Biacore sensor chip. (c) values, determined from fitting the association data in (b) to a pseudo-first order process with a nonspecific component, were replotted vs. sFRP-5 concentrations, and a value of 32 ± 3 nM was determined by nonlinear regression analysis. The data represent mean ± SEM of three independent experiments. .

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