Mediators of Inflammation

Research Article

Involvement of the IL23/Th17 Pathway in the Pathogenesis of Tunisian Pemphigus Foliaceus

Table 2

Primary information of genotyped SNPs in Th17/IL23 pathway’s genes.


Gene SNPs	Base change	MAF	Chromosome regions	Localization	Primers	Enzyme

IL23R rs1884444^a	G/T nonsens	0.47	Chr1 1p31.3	Exon2	F:Fam^✪ TGCTCTGTTTCCTTCCTTCC R1:CATCCCATTGAATAGTGACC R2:T₄CATCCCATTGAATAGTGACA	—
IL23R rs7517847	G/T	0.36		Intron6	F:ATTTCTGGATGCCCTTTCCT R:ACATGAATTTGAGGGGCCTA	BbvI
IL23R rs11209026	A/G Arg-gln	0.022		Exon8	F:TTAGACAACAGAGGAGACATTGGA R:CATGTAGTCTAAATCAGAAAACAGAAA	Hpy188I
IL23R rs10889677	A/C	0.39		3UTR	F:TGCTGGGCCATATGATAAGC R:TCCACCTTCGGGACCTTAAT	MnII
RORγt rs9645406	T/C		Chr1 1q21.3	Intron	F1:T4CCTCACAGCAAATCTTTTCTC F2:CCTCACAGCAAATCTTTTCTT R:NED^✪AAAAACCGCTGTAGGGATCA	—
IL17A rs2275913	A/G	0.29	Chr6 6p12.2	5UTR	F:ATTTCTGCCCTTCCCATTTT R:TTGTGCCTGCTATGAGATGG	EarI
IL17A rs3748067	T/C	0.077	Chr6 6p12.2	3UTR	R:GGGGCGAAAATGGTTACGAT F:AAGGCCCCTCAGAGATCAAC	ApoI
IL17F rs763780	C/T	0.093	Chr6 6p12.2	Exon3	F:TGGGTAAGGAGTGGCATTTC R:CTGCATCAATGCTCAAGGAA	NlaIII
IL17RA rs4819554	G/A	0.233	Chr22 22q11.1	5UTR	F:TGAAATGTGTAATTCGCTGGC R:TGCTTTCCTTGGCTTTGCTT	PvuII
TNFα rs1800629	A/G	0.090	Chr6 6p21.33	5UTR	F:AGGCAATAGGTTTTGAGGGGCAT R:TCCTCCCTGCTCCGATTCC	NcoI
STAT3 rs744166	C/T	0.482	Chr17 17q21.2	Intron	F:GCTGGAGTACAAACCCTGAA R:TGGTATTCAGATGGCGGTCA	AluI

Genotyped using the AS-PCR method.