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Mediators of Inflammation
Volume 2018, Article ID 8352727, 9 pages
Research Article

Lactate as a Potential Biomarker of Sepsis in a Rat Cecal Ligation and Puncture Model

1Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China
2Weil Institute of Emergency and Critical Care Research, School of Medicine, Virginia Commonwealth University, Richmond, VA, USA
3Department of Emergency Medicine, Virginia Commonwealth University, Richmond, VA, USA

Correspondence should be addressed to Longyuan Jiang; moc.361.piv@4691ylj and Wanchun Tang; gro.htlaehucv@gnaT.nuhcnaW

Received 19 May 2017; Revised 18 November 2017; Accepted 13 December 2017; Published 7 March 2018

Academic Editor: Tânia Silvia Fröde

Copyright © 2018 Xiaozhu Zhai et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We attempted to investigate whether blood lactate is a useful biomarker for sepsis in a rat cecal ligation and puncture (CLP) model. Male Sprague-Dawley rats underwent approximately 75% cecum ligation and two punctures to induce high-grade sepsis. A lactate of 1.64 mmol/L (Youden score of 0.722) was selected as the best cutoff value to predict the onset of sepsis after CLP exposure; 46 of 50 rats who survived 24 hours after the CLP were divided into the L group (lactate < 1.64 mmol/L) and M group (lactate ≥ 1.64 mmol/L). In the M group, the animals had significantly higher murine sepsis scores and none survived 5 days post-CLP, and the rate of validated septic animals, serum procalcitonin, high mobility group box 1, blood urea nitrogen, alanine transaminase, cardiac troponin I, and the wet-to-dry weight ratio were significantly higher compared to the L group. Worsen PaO2/FiO2, microcirculations, and mean arterial pressure were observed in the M group. More severe damage in major organs was confirmed by histopathological scores in the M group compared with the L group. In conclusion, lactate ≥ 1.64 mmol/L might serve as a potential biomarker to identify the onset of sepsis in a rat CLP model.