The Role of Regulatory T Cell in Nontypeable Haemophilus influenzae-Induced Acute Exacerbation of Chronic Obstructive Pulmonary Disease
Immunohistochemical detection of Foxp3 and RORγt. Lung tissue sections were immunostained with antibodies to Foxp3 and RORγt. Color shade in (a–h) indicated the intensity of protein expression. Black arrows in (f, g, and h) represented high level of RORγt expression. Mean grey level of each section was analyzed via Motic Images Advanced 3.2, shown in (i and j). Higher grey level value meant lower positive expression. in each group. Data was expressed as mean ± SD. Analysis of differences among groups was conducted with one-way ANOVA-LSD. versus NC; and versus CS.