Intrauterine Malnutrition Reduced Long Leptin Receptor Isoform Expression and Proinflammatory Cytokine Production in Male Rat Pulmonary Endothelial Cells Stimulated by Lipopolysaccharide

Figure 7

Involvement of the p38 kinase and NF-κB pathways in the regulation of Ob-R expression on LPS-induced Ob-R expression in pulmonary endothelial cells. Endothelial cells were harvested 6 h after stimulus with LPS and/or leptin to quantify the activation of the p38 MAPK and NF-κB pathways using Western blotting. The graphs represent the band intensities determined by densitometric analyses and normalized to the total amount of β-actin (NF-κB) and β-actinin (pp38) present in each lane. Cells were obtained from 8 male Wistar rats selected randomly from 5 different litters per group. The results are presented as the means ± SEM, .

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