CD38 Deficiency Promotes Inflammatory Response through Activating Sirt1/NF-κB-Mediated Inhibition of TLR2 Expression in Macrophages

Figure 4

Sirt1/NF-κB regulates TLR2 expression in RAW264.7 cells. The expressions of Sirt1, TLR2, NF-κB p65, and acetyl-p65 (Ac-p65) proteins were detected by Western blot (a) and quantitatively determined for Sirt1 (b), TLR2 (c), NF-κB p65 (d), and NF-κB Ac-p65 (e) in RAW264.7 cells treated with Sirt1 antagonist resveratrol (RSV) or Sirt1 inhibitor nicotianamine (NAM). GAPDH was used as an internal control. The mRNA expressions of TLR2 were examined by qRT-PCR in RAW264.7 cells treated with various concentrations of NF-κB inhibitor PDTC (f), and the protein levels of TLR2 were determined by Western blot treated with PDTC in RAW264.7 cells (g). The data represent the . from three independent experiments, and compared with the vehicle group.

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