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Figure 5: Autophagy inhibitor 3-Ma treatment inhibits M. pneumonia-induced BEAS-2B cells injury. BEAS-2B cells treated with 3-Ma (4 mM) for 2 h prior stimulated with 20 ng/ml MP for up to 24 h. (a and b) Apoptosis of BEAS-2B cells was measured with FITC-annexin V/PI staining and flow cytometry. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . versus ctrl group; versus MPP group. (c–e) Inflammatory cytokines IL-6, IL-1β, and TNF-α expression in cell supernatant were measured by ELISA. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . versus ctrl group; versus MPP group. (f) TGF-β expression in cell supernatant was measured with ELISA. Data are presented as the mean ± SD with Tukey–Kramer multiple comparison test analysis. . versus ctrl group; versus MPP group. (g–j) Fibrotic correlation factor α-SMA, collagen I, and collagen III expression in cell were measured with western blot. . versus ctrl group; versus MPP group.