Dynamic DNA Methylation Changes of Tbx21 and Rorc during Experimental Autoimmune Uveitis in Mice
Table 3
Methylation levels of Tbx21, Gata3, Rorc, and Foxp3 in the retinas of EAU mice at different time points.
Gene
CpG site
Naïve methylation level (%)
EAU 7th-day methylation level (%)
EAU 14th-day methylation level (%)
EAU 21st-day methylation level (%)
EAU 28th-day methylation level (%)
Tbx21
CpG_1
9.92 ± 2.27#
7.58 ± 1.74#
4.67 ± 2.40#
20.00 ± 3.70#
14.92 ± 8.14#
CpG_3
8.07 ± 2.09
9.66 ± 4.07
6.17 ± 2.80
5.58 ± 6.42
5.00 ± 7.48
CpG_5
6.58 ± 4.59
3.58 ± 3.00
3.67 ± 2.86
4.25 ± 0.41
5.67 ± 4.08
CpG_6, 7
4.25 ± 1.41
2.50 ± 2.35
2.42 ± 1.43
4.53 ± 1.02
4.10 ± 2.48
CpG_8
7.58 ± 1.07
7.67 ± 1.17
6.92 ± 1.88
8.75 ± 0.99
9.25 ± 1.55
Gata3
CpG_1
8.00 ± 2.28
7.00 ± 6.56
8.33 ± 3.43
5.50 ± 1.87
10.33 ± 2.94
CpG_2
17.00 ± 7.40
33.17 ± 21.32
26.42 ± 13.28
16.17 ± 8.45
19.50 ± 4.85
CpG_6, 7, 8
13.00 ± 3.11
15.33 ± 6.89
14.83 ± 8.04
12.75 ± 5.52
17.00 ± 5.68
CpG_9
2.58 ± 1.24
1.50 ± 1.97
2.00 ± 1.45
1.50 ± 1.22
1.00 ± 0.71
CpG_10, 11
14.58 ± 1.69
9.60 ± 3.58
22.92 ± 10.87
26.80 ± 24.51
30.58 ± 15.79
CpG_14
2.92 ± 3.01
4.40 ± 5.18
3.75 ± 3.17
2.42 ± 1.36
1.17 ± 1.16
CpG_16
5.57 ± 1.97
2.40 ± 3.31
4.75 ± 2.54
5.50 ± 0.77
4.75 ± 2.82
Rorc
CpG_1
28.58 ± 3.90#
16.92 ± 4.98#
10.00 ± 8.99#
23.25 ± 4.96#
25.00 ± 2.92#
CpG_2
72.60 ± 4.77
64.75 ± 1.29
74.25 ± 3.72
71.67 ± 5.32
56.25 ± 22.09
CpG_3, 4
11.25 ± 4.83
24.50 ± 30.21
15.42 ± 3.64
21.46 ± 20.89
56.50 ± 3.32
CpG_5
38.75 ± 5.96
36.41 ± 9.09
33.08 ± 8.37
46.30 ± 11.80
51.00 ± 11.34
CpG_6
36.75 ± 4.77
31.92 ± 2.15
31.25 ± 9.79
31.08 ± 4.43
33.50 ± 3.41
Foxp3
CpG_2
81.58 ± 5.15
78.83 ± 9.30
79.00 ± 3.78
80.00 ± 5.48
85.38 ± 5.50
CpG_3
89.33 ± 19.74
97.08 ± 5.12
98.75 ± 1.99
99.80 ± 0.45
90.50 ± 19.00
CpG_5
62.50 ± 33.89
64.25 ± 23.35
73.75 ± 21.14
71.00 ± 18.57
75.33 ± 4.51
# indicates the data of the group showing a statistically significant (,, and ) difference compared with other groups among the five groups (one-way ANOVA followed by Bonferroni correction was used to compare the methylation levels in multiple groups).
