Neostigmine Attenuates Proinflammatory Cytokine Expression in Preoptic Area but Not Choroid Plexus during Lipopolysaccharide-Induced Systemic Inflammation

Table 4

Effect of lipopolysaccharide (LPS; 400 ng/kg; iv.) and acetylcholinesterase inhibitors: donepezil (2.5 mg/animal; iv.) and neostigmine (0.5 mg/animal; iv.) injections on the relative gene expression (mean ± SEM; animals per group) of proinflammatory cytokines and their corresponding receptors in the ovine choroid plexus.

Gene	Choroid plexus
	Control	Don.	Neo.	LPS	Don. + LPS	Neo. + LPS
IL-1β	1 ± 0.2^A	0.6 ± 0.1^A	0.5 ± 0.1^A	4.3 ± 1.5^B	4 ± 0.7^B	5.7 ± 1.4^B
IL1R1	1 ± 0.2^A	0.8 ± 0.1^A	0.9 ± 0.1^A	2.1 ± 0.5^B	2.2 ± 0.3^B	2.1 ± 0.2^B
IL1R2	1 ± 0.2^A	0.6 ± 0.1^A	1.1 ± 0.3^A	2.9 ± 0.7^B	3.7 ± 0.7^B	4.4 ± 0.9^B
IL-1RA	1 ± 0.1^A	1 ± 0.1^A	1.3 ± 0.1^A	9.4 ± 0.6^B	12.4 ± 1.3^B	12.7 ± 2.5^B
IL-6	1 ± 0.4^A	0.9 ± 0.1^A	1.2 ± 0.3^A	84 ± 27^B	84 ± 9^B	123 ± 40^B
IL6R	1 ± 0.1^AC	1 ± 0.1^A	1.1 ± 0.0^A	0.8 ± 0.1^BC	0.9 ± 0.1^ABC	0.7 ± 0.1^B
IL6ST	1 ± 0.2^A	0.7 ± 0.1^A	0.9 ± 0.1^A	1.7 ± 0.2^B	2 ± 0.2^B	1.8 ± 0.4^B
TNF	1 ± 0.1^A	0.7 ± 0.1^A	1 ± 0.1^A	1.9 ± 0.6^B	1.6 ± 0.2^B	1.9 ± 06^B
TNFRSF1A	1 ± 0.1^A	0.8 ± 0.1^A	1 ± 0.1^A	1.3 ± 0.1^B	1.4 ± 0.1^B	1.4 ± 0.1^B
TNFRSF1B	1 ± 0.1^AB	0.8 ± 0.1^A	0.8 ± 0.1^A	1.6 ± 0.3^C	1.7 ± 0.3^C	1.4 ± 0.2^BC

IL-1β: interleukin-1β; IL1R1 and IL1R2: interleukin receptor types 1 and 2; IL1RN: interleukin-1 receptor antagonist; IL-6: interleukin-6; IL6R: glycoprotein 130; TNF: tumor necrosis factor; TNFRSF1A and B: tumor necrosis factor receptor types 1 and 2; TLR4: toll-like receptor 4. The gene expression data were normalised to the average relative level of gene expression in the control group of ewes, which was set to 1.0. Different capital letters indicate significant () differences according to a two-way ANOVA followed by Fisher’s post hoc test.