Maternal Plasma Metabolomic Profiles in Spontaneous Preterm Birth: Preliminary Results

Table 2

List of identified metabolites significantly different between the studied groups.

Compound	Group I versus group II				Group Ia versus group II				Group I versus group III				Group II versus group III
	Change (%)	value	AUC	Greater value	Change (%)	value	AUC	Greater value	Change (%)	value	AUC	Greater value	Change (%)	value	AUC	Greater value	CV (%)
Amino acids
Lysine^(p,a)	−8	—	—	—	−13	—	—	—	8	—	—	—	18		0.66		7
Histidine^(n,a)	−17	—	—	—	−22		0.71		−6	—	—	—	13	—	—	—	14
Tryptophan^(p,a)	−12		0.66		−18		0.73		7	—	—	—	22		0.73		15
Fatty acids
Lauric^(n,a)	19	—	—	—	23		0.69		−1	—	—	—	−17		0.68		14
Myristic^(n,a)	31	—	—	—	32		0.71		−3	—	—	—	−26		0.73		22
Hydroxymyristic acid^(n,b)	29	—	—	—	39	—	—	—	−6	—	—	—	−27		0.73		18
Palmotoleic acid^(n,a)	66		0.71		79		0.75		−10	—	—	—	−46		0.80		9
Linolenic acid (C18:3w3)^(n,a)	64		0.69		84		0.77		3	—	—	—	−37		0.75		10
Linoleic acid (C18:2w6)^(p,a)	44		0.69		61		0.76		−3	—	—	—	−32		0.76		10
Oleic acid^(p,a)	46		0.71		68		0.80		−2	—	—	—	−33		0.78		6
Arachidonic acid^(n,a)	22	—	—	—	33		0.70		2	−	−	−	−16		0.69		17
Eicosadienoic (C20:2)^(n,a)	52	—	—	—	61		0.73		16	—	—	—	−23		0.72		18
Docosahexaenoic acid (DHA)^(n,a)	18	—	—	—	30		0.71		−5	—	—	—	−19	—	—	—	17
Docosapentaenoic acid (C22:5w3)^(n,a)	30	—	—	—	46		0.69		−9	—	—	—	−30		0.72		14
Lipids
C16 sphingosine-1-phosphate^(n,a)	−14	—	—	—	−13	—	—	—	−18		0.70		−5	—	—	—	7
Lyso PC (14:0)^(p,a)	−7	—	—	—	−16	—	—	—	27	—	—	—	36		0.70		11
Lyso PC/PC (15:0)^(n,a)	−8	—	—	—	−9	—	—	—	23	—	—	—	33		0.69		13
Lyso PE (18:1)^(p,a)	−7	—	—	—	−8	—	—	—	14	—	—	—	23		0.69		12
Lyso PE (18:2)^(p,a)	−3	—	—	—	−5	—	—	—	25	—	—	—	29		0.71		11
Lyso PC or PC (18:3)^(p,a)	−21	—	—	—	−26	—	—	—	28	—	—	—	61		0.71		26
LysoPE(20:5)^(p,a)	2	—	—	—	−1	—	—	—	76		0.68		72		0.71		28
PS(O-18:0/0:0)^(p,a)	−11	—	—	—	−17	—	—	—	31		0.70		47		0.81		10
TG (triglyceride)^(n,b)	29	—	—	—	28		0.70		−12	—	—	—	−31		0.80		22
Hormones
Progesterone^(p,a)	−8	—	—	—	−11	—	—	—	−33		0.83		−27		0.79		6
Pregnenolone/bolasterone^(p,b)	−5	—	—	—	0	—	—	—	−49		0.85		−46		0.84		8
Pregnenolone sulfate^(p,b)	−33		0.71		−45		0.76		−33		0.75		0	—	—	—	20
17-Hydroxypregnenolone sulfate^(n,b)	−5	—	—	—	−10	—	—	—	−32		0.76		−28		0.74		12
11-Beta-hydroxyandrosterone-3-glucuronide^(n,b)	−46	—	—	—	−42				−50		0.83		−7		0.73		29
Cortisone/aldosterone/prednisolone^(p,b)	0	—	—	—	6	—	—	—	−27		0.80		−27		0.83		8
Cortisol/hydroxycorticosterone^(p,b)	10	—	—	—	20	—	—	—	−20	—	—	—	−27		0.73		5
3b,16a-Dihydroxyandrostenone sulfate^(n,a)	17	—	—	—	26	—	—	—	−30	—	—	—	−40		0.71		21
Testosterone meabolite^(n,b)	−1	—	—	—	−1	—	—	—	−30	—	—	—	−30		0.68		15
Bile acids
Deoxycholic acid^(n,a)	−36	—	—	—	−47	—	—	—	62	—	—	—	154		0.72		30
Glycochenodeoxycholate^(n,a)	−12	—	—	—	−17	—	—	—	145		0.81		180		0.87		6
Chenodeoxyglycocholate/glycoursodeoxycholate/glycodeoxycholate^(p,b)	−40	—	—	—	−51		0.69		61	—	—	—	166		0.80		10
Chenodeoxyglycocholate/glycoursodeoxycholate/glycodeoxycholate^(n,b)	−17	—	—	—	−25	—	—	—	210		0.78		275		0.83		11
Chenodeoxyglycocholate/glycoursodeoxycholate/glycodeoxycholate^(n,b)	−40	—	—	—	−51	—	—	—	25		0.73		109		0.79		6
Glycocholic acid^(p,a)	−11	—	—	—	−12	—	—	—	92	—	—	—	116		0.74		15
TUDCA/TUDCA isomer/taurodeoxycholic acid^(n,b)	−19	—	—	—	−32	—	—	—	74		0.70		116		0.76		7
TUDCA/TUDCA isomer/taurodeoxycholic acid^(n,b)	0	—	—	—	−4	—	—	—	99		0.68		100		0.68		7
Other metabolites
Malonaldehyde^(n,a)	−2	—	—	—	10	—	—	—	11	—	—	—	14		0.71		25
2-Hydroksybutyric acid/3-hydroksybutyric acid^(n,b)	58	—	—	—	89		0.71		22	—	—	—	−23		0.69		29
Malic acid^(n,a)	3	—	—	—	4	—	—	—	−20		0.75		−23		0.79		7
L-2-Amino-3-(1-pyrazolyl)propanoic acid^(p,b)	−10		0.68		−13		0.72		−4	—	—	—	7	—	—	—	4
Carnitine^(p,a)	−19	—	—	—	−23	—	—	—	21	—	—	—	49		0.70		28
D-Fructose/D-glucose^(n,a)	−2	—	—	—	−4	—	—	—	9	—	—	—	12		0.70		7
N,N-Dicyclohexyl-urea^(p,a)	26	—	—	—	23	—	—	—	−51		0.87		−61		0.91		8
Sphingolipid: obscuraminol A/crucigasterin 277^(p,b)	25	—	—	—	9	—	—	—	−26	—	—	—	−41		0.76		16
(4E,8E,10E-d18:3)Sphingosine^(p,b)	49		0.69		64		0.76		12	—	—	—	−25	—	—	—	10
Anandamide (20:l, n-9)^(p,b)	47	—	—	—	59		0.71		15	—	—	—	−22	—	—	—	23
Biliverdin^(p,a)	1	—	—	—	27	—	—	—	−26		0.71		−27		0.73		9

QC CV: coefficient of variation of a metabolite calculated for QC samples; AUC: area under the curve from ROC validation; greater value: corrected value for AUC; value ≤ 0.05; value ≤ 0.01; value ≤ 0.001; value ≤ 0.0001; metabolites found in negative polarity mode; metabolites found in positive polarity mode; metabolites identified by MS/MS spectra and MS fragmentation pattern; metabolites identified putatively by exact mass data and isotopic pattern distribution.