The Inflammatory Response to Enterotoxigenic E. coli and Probiotic E. faecium in a Coculture Model of Porcine Intestinal Epithelial and Dendritic Cells

Figure 1

Schematic illustration of experimental design. (a) IPEC-J2 monocultures were grown as a monolayer on the top surface of Transwell cell culture inserts. Bacteria were added to the IPEC-J2 compartment. (b) MoDC monocultures were cultivated in 12-well cell culture plates. Bacteria were added to the MoDC compartment. (c)–(d) Cocultures of IPEC-J2 cells grown on Transwell inserts and adherent MoDC located in the bottom compartment. In separate approaches, bacteria were added either (c) to the IPEC-J2 compartment or (d) to the MoDC compartment. The lightning flash indicates the localization of the bacterial challenge with either E. faecium or ETEC.

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