The Inflammatory Response to Enterotoxigenic E. coli and Probiotic E. faecium in a Coculture Model of Porcine Intestinal Epithelial and Dendritic Cells

Figure 6

Protein expression (in pg/ml) of (a) IL-1β, (b) IL-8, (c) TGF-β, and (d) TSLP detected by ELISA in supernatants of porcine MoDC after stimulation with either E. faecium (Ecf) or ETEC. In IPEC-J2/MoDC cocultures, Ecf or ETEC was added either to the apical side of IPEC-J2 cells or to the MoDC compartment. In MoDC monocultures, the bacteria were added to the basolateral compartment. Samples were taken at 6 h after addition of bacteria (means ± SEM). independent experiments per bar. Results of the ANOVA are indicated below each graph. Results of post hoc tests are presented in Supplementary Table 5.

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