The Inflammatory Response to Enterotoxigenic E. coli and Probiotic E. faecium in a Coculture Model of Porcine Intestinal Epithelial and Dendritic Cells

Table 1

Oligonucleotide primers and amplicon length of PCR products.

Gene information	Primer sequence	Amplicon length	Accession number	Reference
IL1B1 (interleukin-1, beta 1, Sus scrofa)	(S) 5- CCT CCT CCC AGG CCT TCT GT -3; (AS) 5- GGG CCA GCC AGCA CTA GAG A -3	178 bp		[31]
IL-18 (interleukin-18, Sus scrofa)	(S) 5- ACG ATG AAG ACC TGG AAT CG -3; (AS) 5- GCC AGA CCT CTA GTG AGG CTA -3	205 bp	AF191088.1
NLRP3 (NLR family, pyrin domain containing 3, Sus scrofa)	(S) 5- AGC AGA TTC CAG TGC ATC AAA G -3; (AS) 5- CCT GGT GAA GCG TTT GTT GAG-3	75 bp	NM_001256770.2	[32]
NLRC4 (NLR family, CARD domain containing 4, Sus scrofa)	(S) 5- TGC TCT GAA ACA CCT TGC AT -3; (AS) 5- GCA TAG ATT CCT GCC TCC AG -3	92 bp	XM_013987922.1
CASP13 (caspase-13, apoptosis-related cysteine peptidase, Sus scrofa)	(S) 5- GTG CTA CAG AAA CGC CAT GA -3; (AS) 5- AGG GCA AAG CTT GAG GGT AT-3	150 bp	XM_003129812.6
CASP1 (caspase-1, apoptosis-related cysteine peptidase, Sus scrofa)	(S) 5- CTC TCC ACA GGT TCA CAA TC -3; (AS) 5- GAA GAC GCA GGC TTA ACT GG -3	116 bp	NM_214162	[33]
ASC (LOC100522011) (apoptosis-associated speck-like protein containing a CARD, Sus scrofa)	(S) 5- CCG ACG AGC TCA AGA AGT TT -3; (AS) 5- AGC TCA GCG CTG TAC TCC TC -3	154 bp	XM_003124468.4
IL-8 (interleukin-8, Sus scrofa)	(S) 5- GGC AGT TTT CCT GCT TTC T -3; (AS) 5- CAG TGG GGT CCA CTC TCA AT -3	154 bp	X61151	[34]
TLR4 (toll-like receptor 4, Sus scrofa)	(S) 5- AGA ACT GCA GGT GCT GGA TT -3; (AS) 5- AGG TTT GTC TCA ACG GCA AC -3	180 bp	AB188301
TGF-β (transforming growth factor beta, Sus scrofa)	(S) 5- TGA CCC GCA GAG AGG CTA TA -3; (AS) 5- CAT GAG GAG CAG GAA GGG C -3	164 bp	NM_214015.2
TBP (TATA box binding protein, Sus scrofa)	(S) 5- GAT GGA CGT TCG GTT TAG G -3; (AS) 5- AGC AGC ACA GTA CGA GCA A -3	124 bp	DQ178129	[35]
YWHAZ (tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide, Sus scrofa)	(S) 5- ATG CAA CCA ACA CAT CCT ATC -3; (AS) 5- GCA TTA TTA GCG TGC TGT CTT -3	178 bp	DQ178130	[35]
B2M (beta-2-microglobulin, Sus scrofa)	(S) 5- AAA CGG AAA GCC AAA TTA CC -3; (AS) 5- ATC CAC AGC GTT AGG AGT GA -3	178 bp	DQ178123	[35]
GAPDH (glyceraldehyde-3-phosphate dehydrogenase, Sus scrofa)	(S) 5- ACT CAC TCT TCT ACC TTT GAT GCT -3; (AS) 5- TGT TGC TGT AGC CAA ATT CA -3	100 bp	DQ178124	[35]