Heme Oxygenase-1 Reduces Sepsis-Induced Endoplasmic Reticulum Stress and Acute Lung Injury

Figure 1

Pulmonary endoplasmic reticulum stress was activated during sepsis. Sprague-Dawley rats received cecal ligation and puncture (CLP) surgery or only laparotomy without CLP surgery (sham group). (a) Lung pathology was detected by using hematoxylin and eosin (H&E) staining. (b-c) Levels of the lung injury score and water content in lung tissues. After treatment with CLP for 18 h, lung injury score (b) evaluation was due to the pathology and levels of the water content (c) in lung tissues were analyzed by using W/D method. (d) Levels of endoplasmic reticulum stress-related genes, that is, PERK, eIF2-α, ATF4, and CHOP, were detected using real-time (RT) PCR method. (e) Level of HO-1 was detected by Western blot method, and gray analysis was performed according to the bands. PERK, double-stranded RNA-dependent protein kinase- (PKR-) like ER kinase; eIF2-α, eukaryotic initiation factor 2-α; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein. Data are shown as mean ± SEM (). and ; one-way ANOVA Tukey’s post hoc test.

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