Insulin-Like Growth Factor-I as an Effector Element of the Cytokine IL-4 in the Development of a Leishmania major Infection

Figure 4

Parasitism in response to cytokine treatments and siRNA transfection. Parasitism (median number of parasites per 100 cells) in L. major-infected RAW 264.7 cells (a, b, e, and f) or BALB/c peritoneal macrophages (c and d) following transfection with IGF-I siRNA (a, b, c, and d), Lipofectamine alone (e), or scrambled siRNA (f) along with cytokine stimulation is shown. Promastigote- (a, c, e, and f) or amastigote-infected (b and d) cells transfected with or without siRNA or Lipofectamine were stimulated with IFN-γ (200 U/mL), IL-4 (2 ng/mL), IL-13 (5 ng/mL), and recombinant IGF-I (rIGF-I, 50 ng/mL) for 48 hours. One representative experiment from three independent assays is shown. (ANOVA and Tukey’s tests).

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