Mannan-Binding Lectin Suppresses Peptidoglycan-Induced TLR2 Activation and Inflammatory Responses

Figure 5

MBL was added before or after treatment with PGN to determine the effect on cellular response. (a) The cytokine levels of IL-6. NC bar indicates a negative control; PGN bar indicates that PMA-activated THP-1 cells were stimulated with PGN (200 μg/ml) alone for 24 h; (MBL + PGN) (6 h) bar indicates preincubation with PGN and MBL (10 μg/ml) for 6 h in room temperature and then were added into PMA-activated THP-1 cells for 24 h; PGN (6 h) + MBL bar indicates that PMA-activated THP-1 cells were preincubated cells with PGN for 6 h and then were added MBL for 24 h; MBL (6 h) + PGN bar indicated that PMA-activated THP-1 cells were preincubated cells with MBL for 6 h and then were added PGN for 24 h. All the cells were incubated at 37°C in a 5% (v/v) CO₂ environment. Cell culture supernatants were collected, and IL-6 was measured by ELISA. (b) The cytokine levels of TNF-α. Cell culture supernatants from (a) were collected, and TNF-α was measured by ELISA. Data shown represent three independent experiments. .

(a)

(b)