Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)

Figure 3

Effect of noreugenin or α-hydroxy-butein on neutrophilic apoptosis in carrageenan-induced inflammation in the mouse model of pleurisy. Neutrophils were characterized in flow cytometry by expression of Ly6G and CD11b and absence of F4/80 in their surface. Basal = cells treated with sterile phosphate-buffered saline (PBS, pH 7.6) only. LPS = cells treated with LPS (5 μg/mL) only. NRG = cells treated with noreugenin (5 μM)+LPS (5 μg/mL). AH-BU = cells treated with α-hydroxy-butein (5 μM)+LPS (5 μg/mL). Bars represent the of experiments conducted in triplicate from three to six animals. The values in brackets represent the percentages of inhibition. and compared to the positive control group (LPS); ANOVA/Newman–Keuls test.