High-Fat Diet Alters Immunogenic Properties of Circulating and Adipose Tissue-Associated Myeloid-Derived CD45⁺DDR2⁺ Cells

Figure 2

Increased percentage of myeloid-derived CD45⁺DDR2⁺ cells express MHC II and CD80 in mammary gland-associated adipose tissue (MGAT) and visceral adipose tissue (VAT) of preobese mice compared to myeloid-derived CD45⁺DDR2⁺ cells in MGAT and VAT of lean control mice. (a–c) Flow cytometric analysis of a representative mouse from each group, showing MHC II and CD80 expression in CD11b-expressing CD45⁺DDR2⁺ cells from MGAT, VAT, and peripheral blood (PB) of HFD- and ND-fed C57BL/6 mice at endpoint (9 weeks of postinitiation of diet). For flow cytometric analysis, cells were first gated on single, live cell populations. (d) Graphical representation of flow cytometric analysis, showing the percentage of total CD45⁺DDR2⁺ cells, CD11b-expressing CD45⁺DDR2⁺ cells, and MHC II and CD80 expression on CD11b-expressing CD45⁺DDR2⁺ cells in MGAT, VAT, and PB of HFD- vs. ND-fed C57BL/6 mice. Data are presented as of 5 mice per group. , , and .

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