Research Article

HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway

Figure 3

Effect of HuoXueTongFu Formula (HXTF) on M1 polarization induced by LPS+IFN-γ. RAW264.7 macrophages were incubated during 12 h with the control DMEM solution and LPS+IFN-γ, respectively. Meanwhile, RAW264.7 cells were preincubated with LPS+IFN-γ for 3 h, followed by combined treatment with HXTF for 12 h. (a) Flow cytometry analysis of M1/M2 marker CD86 and CD206 expression. (b) ELISA analysis of M1/M2 gene marker expression on macrophages. All values are expressed as ( experiments). , , , and , comparison of the designated two groups.
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