HuoXueTongFu Formula Alleviates Intraperitoneal Adhesion by Regulating Macrophage Polarization and the SOCS/JAK2/STAT/PPAR-γ Signalling Pathway

Figure 5

Modulation of PPAR-γ activity on macrophage polarization and PPAR-γ nuclear translocation. RAW264.7 cells were preincubated with either rosiglitazone (RSG) (1 μmol/mL) or T0070907 (1 μmol/mL) for 3 h, followed by combined treatment with/without HuoXueTongFu Formula (HXTF) for 12 h. (a, b) Flow cytometry analysis of M1/M2 marker CD86 and CD206 expression in control, HXTF, RSG, and HXTR+RSG group. (c, d) Flow cytometry analysis of M1/M2 marker CD86 and CD206 expression in control, HXTF, T0070907, and HXTF+T0070907 group. (e) Flow cytometry analysis of PPAR-γ nuclear translocation. (f) Typical cell figures of nuclear untranslocation. (g) Typical cell figures of nuclear translocation.

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