Role of SOCS2 in the Regulation of Immune Response and Development of the Experimental Autoimmune Encephalomyelitis

Figure 4

SOCS2 influences the infiltrating immune cell profile in the CNS. The spinal cord from WT and SOCS2^-/- naive or immunized (MOG) mice was harvested and submitted to Flow Cytometry analysis at 14 and 28 dpi. Numbers of CD3⁺CD4⁺ (a), CD4⁺CD25^hi+FoxP3⁺ (b), CD4⁺CD3⁺IFN-γ⁺ (d), CD3⁺CD4⁺IL-17⁺ (e), and CD4⁺CD3⁺IL-10⁺ (f) of total population were assessed by Flow Cytometry using specific antibodies as described in Material and Methods. (c) Proportion of CD3⁺CD4⁺ (Th) and CD4⁺CD25^hi+FoxP3⁺ (Treg). (g) Proportion of CD4⁺CD3⁺IFN-γ⁺ (Th1), CD3⁺CD4⁺IL-17⁺ (Th17), and CD4⁺CD3⁺IL-10⁺ (Th2) cells ( animals per group). Data are representative of three independent experiments and shown as the . , ^# (comparison between WT EAE 14 days and WT EAE 28 days).

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