Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer

Figure 7

MIF deficiency promotes Th17 cytokine profile development and the alternative activation of macrophages in the colon. Transcripts of molecules related to the tumor microenvironment profile were analyzed by RT-PCR. In this figure, we show the expressions of (a) il-18, (b) il-4, and (c) inos and arginase-1 relative to the housekeeping gene β-actin. (d) Representative electrophoresis gels are shown to show differences between groups. (e) Real-time PCR measurement of relative gene expressions of foxp3 and il-17. Data are representative of two independent experiments and are plotted as the means (±SEM), mice per group; , , and .

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