Macrophage Migration Inhibitory Factor Promotes the Interaction between the Tumor, Macrophages, and T Cells to Regulate the Progression of Chemically Induced Colitis-Associated Colorectal Cancer

Table 1

Sequences of the primers used to determine the immune profile of the tumors by endpoint RT-PCR. The TM and amplicon size are also shown.

Primer	Sequence	TM (°C)	Amplicon (bp)
mif F	5-TGCCCAGAACCGCAACTACAGTAA-3	60	218
mif R	5-TCGCTACCGGTGGATAAACACAGA-3
il-17 F	5-TCCCTCCGCATTGACACA-3	60	83
il-17 R	5-ACCGCAATGAAGACCCTGAT-3
il-18 F	5-ACTGTACAACCGCAGTAATACG-3	58	434
Il-18 R	5-AGTGAACATTACAGATTTATCCC-3
tnf-α F	5-GGCAGGTCTACTTTGGAGTCATTGC	70	195
tnf-α R	ACATTCGAGGCTCCAGTGAATTCG-3
il-10 F	5-ACCTGGTAGAAGTGATGCCCCAGGCA-3	56	237
il-10 R	5-CTATGCAGTTGATGAAGATGTCAAA-3
il-4 F	5-CGAAGAACACAGAGAGTGAGCT-3	58	180
il-4 R	5-GACTCATTCATGGTGCAGCTTATCG-3
il-1β F	5-GAGTGTGGATCCCAAGCAAT-3	59	520
il-1β R	5-CTCAGTGCAGGCTATGACCA-3
foxp3 F	5-GGCCCTTCTCCAGGACAGA-3	60	112
foxp3 R	5-GCTGATCATGGCTGGGTTGT-3
arg-1 F	5-CAGAAGAATGGAAGAGTCAG-3	55	250
arg-1 R	5-CAGATATGCAGGGAGTCACC-3
inos F	5-CTGGAGGAGCTCCTGCCTCATG-3	62	449
inos R	5-GCAGCATCCCCTCTGATGGTG-3
β-Actin F	5-TTTGATGTCACGCACGATTTCC-3	60	514
β-Actin R	5-TGTGATGGTGGGAATGGGTCAG-3