Inhibition of Synovial Macrophage Pyroptosis Alleviates Synovitis and Fibrosis in Knee Osteoarthritis

Figure 3

Inhibition of synovial macrophage pyroptosis may alleviate fibrosis in KOA rats. (a) The severity of synovial fibrosis evaluated by the transverse diameters of the right knees. vs. the Normal group; ^# vs. the KOA+AYC group. (b) Collagen deposition was revealed through Sirius Red staining (200×). (c) Semiquantification of Sirius Red staining was evaluated using the average integral optical density (AIOD). The results were expressed as the (). , in comparison with the Normal group. ^#, in comparison with the KOA group. Data were analyzed by ImageJ. (d) Comparison of TGF-β, PLOD2, COL1A1, and TIMP1 protein expressions between groups. , in comparison with the Normal group. ^#, in comparison with the KOA group (). (e) Typical protein bands for each group. (f) The mRNA levels of TGF-β, PLOD2, COL1A1, and TIMP1 between groups.

(a)

(b)

(c)

(d)

(e)

(f)