Angiotensin-(1-7) and Alamandine Promote Anti-inflammatory Response in Macrophages In Vitro and In Vivo

Figure 3

Expression profile for M(LPS+IFN-γ) and M(IL-4) markers in M0 before and after the treatment with Ang-(1-7) or alamandine. (a, b) Real-time PCR analysis of TNF-α, CCL2, and IL-1β levels in M0 macrophages before and after treatment with Ang-(1-7) (10^-7 M) (a) or alamandine (10^-7 M) (b). (c, d) Real-time PCR analysis of YM1, FIZZ1, and MRC1 transcripts expressions in M0 macrophages before and after treatment with Ang-(1-7, 10^-7 M) (c) or alamandine (10^-7 M) (d). Results were obtained by a -test or nonparametric Mann-Whitney test and are expressed as the mean ± SEM of independent experiments.

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