Angiotensin-(1-7) and Alamandine Promote Anti-inflammatory Response in Macrophages In Vitro and In Vivo

Figure 6

In vivo effects of Ang-(1-7) and alamandine treatments on the number of total and elicited cells and macrophage profile in the pleura during pleurisy. Analysis of the numbers of (a) total cells, (b) mononuclear cells, and (c) neutrophils by morphological counting from cytospin slides or by frequency of macrophages (F4/80⁺CD11b⁺) and neutrophils (GR1⁺F4/80^-) by flow cytometry (d) as well as the frequencies of (e) M1, (f) M2, and (g) Mres macrophages in the pleural wash by flow cytometry in nontreated (PBS, , black bars) or LPS-treated animals (), and in the presence of LPS in treated animals with Ang-(1-7)-HPβCD () or alamandine-HPβCD (). Representative dot plots are shown in (d). Results were directly obtained by one-way ANOVA or after logarithmic transformation (f only) and are expressed as the . , compared to PBS; , compared to PBS; , compared to PBS and LPS treatment only, respectively; , compared to PBS and LPS treatment only, respectively.