IFN Regulatory Factor 1 Mediates Macrophage Pyroptosis Induced by Oxidized Low-Density Lipoprotein in Patients with Acute Coronary Syndrome

Figure 7

IRF-1-induced human macrophage pyroptosis requires ROS. (a) Macrophages from ACS patients were cultured with ox-LDL (48 h) and then divided into five groups in which three groups were transfected with pGC-FU-IRF-1, pGC-FU-IRF-shRNA, and pGC-FU-control-shRNA and one group was pretreated with NAC (10 nM, 24 h) and then were transfected with pGC-FU-IRF-1. Intracellular ROS were assessed by DCFH2-DA (200x). indicates vs ACS group (). indicates vs ACS and IRF-1 group (). # indicates vs ACS group (^#). indicates vs IRF-1 group (). (b) Caspase-1 activity was measured by Ac-YVAD-pNA. (c) Cell death was measured by the percentage of LDH release. (d) Protein expression of NLRP3, ASC, caspase-1 p10, IL-1β p17, and IL-18 was measured by western blot. indicates vs ACS group (). indicates vs ACS and IRF-1 group ().

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