Roles of Inflammasomes in Inflammatory Kidney Diseases

Table 2

Expression profiles of inflammasome members in PBMCs of IgAN patients.

	Healthy donors	IgAN patients	value
Gender	F8/M9	F15/M7	0.19
Age			0.38
CREA			0.007
NLRP1 mRNA levels			0.42
NLRP3 mRNA levels			0.014
NLRC4 mRNA levels			0.64
NAIP mRNA levels			0.69
AIM2 mRNA levels			0.68
PYRIN mRNA levels			0.30
IFI16 mRNA levels			0.08
ASC mRNA levels			0.47
Caspase-1 mRNA levels			0.41
IL-1β mRNA levels			0.10
IL-18 mRNA levels			0.49

Verified healthy donors and primary IgAN patients were enrolled under the supervision of the Ethics Review Committee of the First Affiliated Hospital, Sun Yat-sen University (Guangzhou, China), and this study was conducted in accordance with the guidelines proposed in the Declaration of Helsinki. None of the patients had been treated with steroids and/or immunosuppressive drugs within one year nor did they show clinical infection symptoms at the day when blood samples were taken. PBMCs from venous blood with anticoagulant EDTA-K2 were enriched and subjected to RNA extraction. Gene expression was analyzed with real-time PCR analysis and calculated with the 2^-ΔΔCt method, using GAPDH as the internal control. Sequences and primers for each genes were acquired from the NCBI database (https://www.ncbi.nlm.nih.gov). All statistical assessments were two-sided using a significance value of (indicated as ) and (indicated as ).