Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis

Figure 4

The effect of TRAF2 inhibition on TWEAK-induced cell fates of keratinocytes. Human primary keratinocytes were cultured in vitro and received 48 h stimulation with TWEAK (100 ng/ml). TNFR2-overexpressing keratinocytes were constructed using a retroviral vector. Some cells were pretransfected with siRNA against TRAF2 or a control target. (a) TRAF2 protein was detected by Western blotting. (b) Apoptotic cells were quantified by flow cytometry. (c) The ratios of apoptotic cells were compared between different groups. (d) The proliferation of keratinocytes was quantitated by spectrophotometry, followed by comparison across groups. Data were obtained from five independent experiments. Representative images are shown. ns: not significant.

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