Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis

<div>The effect of TWEAK/Fn14 interaction on TNFR1-dependent downstream signals in normal keratinocytes. Primary human keratinocytes were cultured <i>in vitro</i> and then received 48 h stimulation with TWEAK (100 ng/ml). Some cells were pretransfected with Fn14 or control siRNA. (a) The expression of TRADD and caspase-8 was analyzed in cells via immunofluorescent staining. (b) The expression of TRADD and caspase-8 was detected in cell lysates via Western blot. (c, d) The band intensities of Western blots were measured with ImageJ software. Data were obtained from five independent experiments. 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Mediators of Inflammation

fig5

Figure 5

Figure 5: Tumor Necrosis Factor (TNF) Receptor Expression Determines Keratinocyte Fate upon Stimulation with TNF-Like Weak Inducer of Apoptosis 