Interleukin-12p35 Deficiency Reverses the Th1/Th2 Imbalance, Aggravates the Th17/Treg Imbalance, and Ameliorates Atherosclerosis in ApoE-/- Mice
Effect of rIL-35 on the activity of CD4+ T cells. (a, b) The frequency of Th1, Th2, Th17, and Treg in the ApoE-/- and ApoE-/-+rIL-35 groups was measured by flow cytometry; for each group. (c) The serum IFN-γ, IL-4, IL-17, and IL-10 levels were detected by ELISA; for each group. (d, e) The mRNA levels of T-bet, IFN-γ, GATA3, IL-4, RORγT, IL-17, IL-6, TNF-α, Foxp3, and IL-10 in the spleens and aortas of these two groups were analyzed by RT-qPCR. ApoE KO = ApoE-/-, DKO = ApoE-/- IL-12p35-/-; for each group. vs. the ApoE-/- group.