CD4⁺ TSCMs in the Bone Marrow Assist in Maturation of Antibodies against Influenza in Mice

Figure 5

BM memory CD4⁺ TSCMs assist in affinity maturation of antibodies in vivo. Transferred CD4⁺ TSCMs from the BM provide help for affinity maturation of antibodies in vivo. 4 × 10⁶ CD19⁺ CD138⁻ CD4⁻ cells (naïve B cells) from OT-II mice SP were transferred with 1 × 10⁵ CD4⁺ CD44^low CD62L^high CD122^high Sca-1^high T cells from the BM or SP of OT-II mice, into CD45.1 mice. One day later, recipients were immunized with 2000 hemagglutination units PR8-OVA in CFA. The blood taken from transferred mice 5 days later was analyzed for anti-HA IgG₁ by ELISA. Data are representative of three independent experiments, means ± SD. .