Dihydrotanshinone I Alleviates Crystalline Silica-Induced Pulmonary Inflammation by Regulation of the Th Immune Response and Inhibition of STAT1/STAT3

Figure 5

DHI regulated T regulatory cells in a mouse model of CS-induced inflammation. Percentage of CD4+Foxp3+ T regulatory cells in the HLN (a, c) and spleen (b, d) was detected by flow cytometry ( to 6 per group). (e, f) Foxp3 mRNA level in the spleen and lung was assayed by qRT-PCR ( to 6 per group). (g) TGF-β mRNA level in lung tissues was assayed by qRT-PCR ( to 6 per group). Data are presented as the . ; . All experiments were performed in triplicate.

(a)

(b)

(c)

(d)

(e)

(f)

(g)