Integrated Functional Analysis of the Nuclear Proteome of Classically and Alternatively Activated Macrophages

Table 1

Summary of 2DE/MS results from nuclear fractions of M1 and M2 macrophages.

Proteomics profile	Fold change	M1 vs. M0		M2 vs. M0		M2 versus M1
		Number	Max/Min	Number	Max/Min	Number	Max/Min
Increased abundance	≥1.5	37	10.33	34	3.24	89	6.23
Decreased abundance	≥-1.5	108	-7.32	71	-3.63	50	-6.84
Increased SNO (-ve RoR)	≥-1.5	42	-7.88	31	-4.70	38	-4.57
Decreased SNO (+ve RoR)	≥1.5	36	6.93	50	4.27	66	5.73
Increased phosphorylation	≥1.5	164	6.93	118	100	40	3.90
Decreased phosphorylation	≥-1.5	9	-7.88	46	-100	122	-100

Shown are the numbers of protein spots that exhibited protein differential abundance, SNO modification, or phosphorylation in nuclei of M1 vs. M0, M2 vs. M0, and M2 vs. M1 macrophages (fold change: , ). These protein spots were subjected to MALDI-TOF/TOF MS for protein identification, and complete proteome datasets are presented in S1 Table.