Hemorrhagic Shock Sensitized the Diaphragm to Ventilator-Induced Dysfunction through the Activation of IL-6/JAK/STAT Signaling-Mediated Autophagy in Rats

Figure 3

Roles of IL-6/JAK/STAT signaling in short-term MV-induced VIDD in HS animals ( in each group). (a, b) Western blots for JAK/STAT, the downstream signaling of IL-6, in the diaphragm after MV in HS animals. (c, d) Western blots for JAK/STAT signaling after IL-6 mAb administration. Western blots for (e, f) JAK/STAT signaling and (g, h) LC3B-I/II expression after JAK/STAT inhibitor ruxolitinib (Rux) treatment. (i) IL-6 expression in the diaphragm after Rux treatment. (j–m) Effects of Rux on diaphragm atrophy and weakness. Measurements were repeated three times for each sample. MV = mechanical ventilation; HS = hemorrhagic shock. vs. the HS+MV group.

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