Anti-Atopic Dermatitis Effect of Seaweed Fulvescens Extract via Inhibiting the STAT1 Pathway

Figure 5

Effects of SF on the mRNA expression and production of inflammatory cytokines in HaCaT keratinocytes. (a) Cell viability was determined using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. Cells were seeded in 96-well microplates at cells/well, and various concentrations of SF were added to each well for 24 h. The mRNA expression levels (b, d) and production of inflammatory cytokines (c, e) were determined in HaCaT keratinocytes. Total RNA was isolated from the cells treated with SF for 6 h, and quantitative reverse transcription-polymerase chain reaction was performed. The production of inflammatory cytokines was measured by ELISA. Proinflammatory cytokine levels were measured in the culture supernatants from cells treated with SF (10, 25, and 50 μg/mL) and TNF-α and IFN-γ (each 10 ng/mL) for 24 h. Values represent the of three independent experiments. and versus the Df group; , , and versus the control group.

(a)

(b)

(c)

(d)

(e)