Mast Cell Degranulation Decreases Lipopolysaccharide-Induced Aortic Gene Expression and Systemic Levels of Interleukin-6 In Vivo

Figure 1

Mast cell degranulation in LPS-injected wild-type mice at 24 hours: serum levels IL-6 and aortic expressions of IL-6, TLR-2, TLR-4, COX-1, COX-2, TNF-α, and SOCS-1 mRNA at 24 hours. Wild-type mice 24 hours after injections with either saline, compound 48/80 (systemic mast cell degranulating agent), LPS, or LPS+C48/80. (a) LPS-induced serum IL-6 was inhibited by the administration of C48/80 ( per group). Aortic mRNA expression of IL-6 (b), TLR-2 (c), TLR-4 (d), COX-1 (e), COX-2 (f), TNF-α (g), and SOCS-1 (h) determined by qPCR. The expression levels in the saline group were normalized to 1. LPS-induced aortic expression of IL-6 was inhibited by the administration of C48/80. SOCS-1 expression, an intracellular inhibitor of IL-6 signaling, was highest in the LPS+C48/80 group ( in the saline group, in the C48/80 group, in the LPS group, and in the LPS+C48/80 group). and .

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