Coronary Artery Disease Is Associated with an Increased Amount of T Lymphocytes in Human Epicardial Adipose Tissue

Figure 1

Flow cytometry: gating strategy. Doublets were excluded, lymphocytes were gated according to SSC properties and CD45 positivity, and T (CD3+) and CD3- cells were assessed. B lymphocytes (CD19+CD3-CD45+ cells) and NK cells (CD16/56+CD3-CD45+ cells) were gated from CD3- cells. T helper lymphocytes (CD4+CD3+CD45+ cells), T cytotoxic lymphocytes (CD8+CD3+CD45+ cells), and NKT cells (CD16/56+CD3+CD45+ cells) were gated from T (CD3+) cells.