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Figure 2: IL-4 promotes myogenesis by up-regulating expression of myocyte-specific marker. (a) Levels of myogenin, MYF6, CDK5 and MyHC protein expression at the time indicated were analyzed. (b) Morphology of C2C12 cells at the indicated day. (c) Cell lysates were harvested at the time indicated and IL-4R and GATA3 was analyzed (n = 3). and v.s control at day 0, #p < 0.05 v.s IL-4 at day 0, p < 0.05 v.s control at the same day. (d) IL-4 in culture medium harvested at the day indicated was measured by ELISA. v.s control at day 0.