Figure 4: IL-4 promotes insulin signaling and GLUT4 translocation in C2C12 myocytes. Myocytes were transferred to serum free medium for 3 hr, followed by exposure to IL-4 and/or insulin for 30 min. (a) Cell lysate were harvested at indicated time, and levels of p-Akt and p-GSK-3β were analyzed (n = 3). v.s 0 min, #p < 0.05 v.s INS group at the same time point. (b) GLUT4 translocation and (c) glucose uptake was assayed as described in Methods (n = 3). v.s control, #p < 0.05 v.s INS.